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Posted by artninja1988 2 days ago

The Millennium Problems for Biology(millenniumproblems.bio)
160 points | 119 comments
doctoboggan 2 days ago|
Someone should make the millennium problems for AI alignment so the frontier labs will actually try to solve that problem.
aleph_minus_one 2 days ago||
> Someone should make the millennium problems for AI alignment so the frontier labs will actually try to solve that problem.

Rather: Someone should make the millennium problems for human alignment so the top researchers will actually try to solve that problem. :-)

a83ndoija9isjd 2 days ago||
They are already human-aligned. That is the entire issue.
busssard 1 day ago||
because run by humans? i would not consider them aligned with human goals per se..
hodgehog11 2 days ago|||
A group already (sort-of) has for the broader theory: https://arxiv.org/abs/2604.21691

It's a good list, but "alignment" is much more targetted. I attended a workshop with researchers from OpenAI and Anthropic also present, where the objective was to hash out what the problems in alignment even are. This, it turned out, was extraordinarily difficult.

The problems with alignment are to do with how we define and search for this vague notion when it is mathematically ill-posed at present.

wise_blood 1 day ago|||
I was gonna write "Implement the three laws of robotics", but then I remembered that the point of the short stories is that there are a number of loopholes...
mettamage 1 day ago|||
For 1 million dollars?

I hope you are right.

vjvjvjvjghv 2 days ago|||
The only thing the frontier labs are interested in is their trillion dollar IPOs.
daveguy 2 days ago|||
You'd think that believing they are going to destroy humanity would motivate them.
an0malous 2 days ago|||
If you ignore what people say and instead observe what they do, the world makes a lot more sense.
throwup238 1 day ago||||
Some ~~people~~ corporations just want to see the world burn.
alansaber 2 days ago|||
They're for-profit corporations above all.
mmooss 2 days ago|||
They are human institutions above all. Saying 'we're a corporation' doesn't at all absolve them from human traits, consequences, and responsibilities.
pixl97 2 days ago|||
Corporations are really the embodiment of Moloch.
aaron695 2 days ago||
[dead]
pfisherman 2 days ago||
Biology already has numerous “Millennium Problems” and the rewards are much more than $1M. For example, reverse Alzheimer’s Disease. Or less ambitious develop high fidelity in vitro and animal models of human disease.
phreeza 2 days ago||
Those don't have the property of being easily verified, which the problems proposed here do. I think that's a smart idea because it's a way to capture quick PR seeking AI-lab dollars for quite useful outcomes.
pfisherman 2 days ago||
Being easily verifiable does not elevate something to the level of being a grand challenge. It really drives home the point that biology is not math or coding.

I guess to me the grandiosity here feels fake and unearned - like they vibe slopped it together without much input in the way of deep thought or expertise.

For example, synthesize arbitrary dna sequences > 3000 nt in length with error rate < 0.001 at > 95% purity. Easily verifiable, beyond the frontier, and generally useful.

phreeza 2 days ago|||
I agree with you there are other much grander challenges, but apart from that I don't think the list is horrible, because as I said it's incentive-aligned with the current moment we are in. Your suggestion would definitely be a good addition to the list for sure.

I think some of the items on the list are also way less likely to be worked on (like cryogenics) than others, because they really require some heavy infrastructure to iterate. My money for which of these gets cracked with help of an AI would be rubisco, that can be quite effectively worked on in a closed loop lab fashion with a standard lab or CRO.

pixl97 2 days ago|||
>that biology is not math or coding

Biology is a really really big field. Many problems in biology are that of math and coding. Just as a simple example look at the golden ratio in living organisms. Biology follows a lot of different algorithms because they are energy efficient and come with massive secondary benefits.

root_axis 2 days ago||
> Just as a simple example look at the golden ratio in living organisms

What is this example meant to illustrate?

pixl97 2 days ago|||
The golden ratio is one of those things you can study for years and be a amazed by.

>The golden ratio (≈ 1.618) and its related golden angle (137.5°) show up in nature because they maximize space, exposure, and flow.

The golden ratio is an algorithm, but it is also a natural law that systems of many different scales follow. Wherever we look we find examples of it. Without this algorithm the world is a much harder place to explain.

What many scientists starting to look for is these algorithms we have not identified in natural systems as an explanatory means of their workings. Also because they are algorithms they are things we can put into computer systems to increase efficiency, or may naturally emerge from evolutionary learning systems like AI in what we call emegence.

esafak 2 days ago||
The golden ratio in nature is numerology. Biology is beautiful enough in itself without having the golden ratio shoehorned into it. https://pmc.ncbi.nlm.nih.gov/articles/PMC10792139/
rolph 2 days ago|||
agreed, the golden ratio in the case of biological structure is a consequence of geometric progression of progressive assembly, its not a driver.

the better direction in my opinion, is the mutual reinforcement of coupled dynamic systems, that ends up giving us metabolism.

pixl97 2 days ago|||
Did you even read what you linked to, or did you just read the headline?

>There is no convincing evidence that the golden ratio is linked to idealized human proportions or facial beauty. There is currently no evidence to support the use of the golden ratio in orthognathic or facial aesthetic/reconstructive surgical planning or analysis of results.

This is about peoples faces. What I listed was about things like available surface area for things like chemical reactions and mixing, you know things that are mathematically calculatable. Much in the same manner a hexagon is an optimal low energy shape, hence we see lots of them in nature too.

SiempreViernes 2 days ago|||
I think it illustrates that there are cool things to find beneath the lamp post of math and coding.
pixl97 2 days ago||
Not just cool things. But real things that effect physics.
jszymborski 2 days ago||
I think those are better described as problems of medicine whereas the problems here are indeed about fundamental biology
daoboy 2 days ago||
This is all well outside my area of expertise, but my impression is that Michael Levin's work with bioelectricity is on the cusp of #6 Somatic limb regeneration.

SMEs please correct the record if I'm mistaken.

jodacola 2 days ago||
Unfamiliar with Levin’s work, but:

There’s also this study [0] I read recently, along a different path of using known growth factors and proteins to kick off regeneration.

[0] https://www.nature.com/articles/s41467-026-72066-8

gavinray 2 days ago||
ECM (Extracellular Matrix) has been studied for similar properties:

https://www.biorxiv.org/content/10.64898/2026.08.05.742898v1...

"Extracellular matrix particle treatment induces digit regeneration in soft-tissue preserved amputation (SPA) model of adult mice"

pixl97 2 days ago||
Everyone interested in biology and intelligence should look up Michaels work, it is amazing because he's ignored a lot of assumptions that are held by the community and come up with new and interesting tests.

Also his work on algorithmic intelligence in cells and the mathematical foundation of intelligence will change many fields, including AI, if it can be empirically tested.

usernametaken29 2 days ago||
Wait a minute:

> Cryopreservation + Demonstrate the ability to cryopreserve and recover live wild-type mice with high viability. Specifically, demonstrate the reversible cryopreservation of live, intact, wild-type adult mice in a whole-body frozen or vitrified state. The mice must remain frozen or vitrified for at least 24 hours, must be recovered with >99% viability, and must not suffer any permanent organ damage or bodily harm. Somatic genetic engineering is discouraged but permitted. All experiments must be conducted with ethics approval.

This has been done in the 50ies, freezing and thawing mice with microwaves. IIRC the recovery rate was about 74% with no observed side effects. The research was given up on because in this specific case a mouse is a bad biological model. Thawing agents must scale cubically with size. The author says that at approximately the size of a cat you can’t quickly enough evaporate all the agent because the energy required will simply burn the tissue.

TLDR: it’s possible, it’s been done, it could be perfected if necessary, it does NOT scale beyond mice

shevy-java 2 days ago||
> Demonstrate the emergence of life from chemical precursors in a laboratory setting.

> Specifically, demonstrate the unassisted emergence of self replicating RNA- and protein-based cells from a plausible primordial soup with a plausible energy source. A “cell” may be any compartment with a defined boundary.

So, right now, nobody can explain how life originated. Proving that aminoacids, DNA or RNA form, does NOT mean that this is equal to life. This is a problem that the whole field has - it still can not explain how life originated. Showing that individual components can arise spontaneously, is not the same as showing e. g. how a cell formed and so forth. Where does the encoding problem fit into any of that, for instance? You need to prove how you can assemble systems. Just having a working ribosome does not connect it to DNA as a genetic backup system; and RNA tends to be unstable. Even when you have assumptions how this works together, you need to prove that this is how things originate(d). Nobody has done so since decades. It is an unsolved problem.

bonsai_spool 2 days ago||
This is a collection of pet projects by folks who are not distinguished biologists. Interesting, maybe, but not to be placed on the same pedestal as the mathematics Millenium Prize of a similar name.

Arguably, the origin of life is a question for all time -- no way that a VC webpage is going to change whether someone takes that problem on, and it will be commercialized with big-name capitalists instead of 'FutureHouse' should it ever be developed in the lab.

hyperbovine 2 days ago||
This. Part of the allure of the Clay Millenium prizes is that some of the best minds in the field came up with them. Let’s hear what Eric Lander, Jennifer Doudna, Aviv Regev, Feng Zhang, David Baker, Robert Weinberg, George Church, Bert Vogelstein, etc have to say.
dannykwells 1 day ago||
Also Bingo.
PaulKeeble 2 days ago||
Its much harder to produce a good fundamental list of biology problems because we are no where near as far along, so many basic things are not understood. There are still many unknown unknowns and these particular problems are limited to the realm of verifiable in a lab, which rules out much of what makes biology interesting and hard to study, and that is the organisms themselves and the interactions between their cells. The petri dish differs from the rat differs from the human and that is a part of what makes biology harder to progress.

None of these is likely the root to the wide array of chronic illnesses we can't treat and those seem like the next step to really put a lot of research into given how many people suffer from them and where we are today they seem achievable with the right investment.

shevy-java 2 days ago|
> The petri dish differs from the rat differs from the human and that is a part of what makes biology harder to progress.

They all use DNA.

But I agree that nobody has shown in the lab how any of this leads to a genetic system that can self-reproduce reliably and assemble biomolecules/metabolites. This is a missing link. Just showing how RNA or amino acids arise, says nothing about any genetic system. They can not even answer whether RNA or DNA viruses existed first; and whether these existed before organisms/cells did.

throwup238 1 day ago|||
> Just showing how RNA or amino acids arise, says nothing about any genetic system. They can not even answer whether RNA or DNA viruses existed first; and whether these existed before organisms/cells did.

Actually, we have quite a bit of evidence to evidence pointing at the order of development based on how strongly conserved some critical features of biochemistry.

The core component of ribosomes (which make proteins) is made from RNA called ribozymes. Other things like RNase P, self-splicing introns, the RNA cores of the spliceosome, and the fact that most central metabolic cofactors (ATP, NAD, FAD, coenzyme A) have ribonucleotide handles points to RNA as the initial building block, before viruses or archae or prokaryotes were even born.

It wasn’t until ribonucleotide reductase gets involved that RNA becomes DNA. DNA swaps uracil for thymine making the genome more error-resistant and a double helix to get stability over reactivity, as a clear evolutionary strategy because DNA doesn’t form like RNA does.

The core of the RNA world hypothesis is a ton of experiments that find that RNA and amino acids are stable in many different chemical conditions which cover a wide range of what the early earth would have looked like along with observed amino acids on outer space objects like comets and asteroids. What we don’t have is an experiment demonstrating how RNA becomes self replicating with the addition of amino acids, which would be one of the last steps to showing how life likely formed.

HarHarVeryFunny 2 days ago||||
The goal of the suggested "origins of life" problem, as written, isn't to guess at history - it's to create some similar plausible self-emergent system in the lab.

Seems a bit of an ambitious goal for a language model though! There are presumably dozens of steps before you get to an RNA-based full-blown cell, and it's only been very recently that humans have managed to make an artificial self-replicating "cell" (container) of any type in the lab.

pixl97 2 days ago|||
>They all use DNA.

I really really recommend looking up Michael Levins bio work on intelligence at the cellular and organ level.

His most recent work is starting to touch on some pretty far out concepts. And I should note, that he does not state they are true or not, but they are attempting to make empirical methods on testing them.

One of the concepts they are looking at is "math as an actual thing" and that some simple algorithms have new deeper behaviors that we're just finding now. The hypothesis is that over the eons life has probed mathematics at scales humanity cannot even begin to imagine and is exploiting this deep algorithmic efficiency in accomplishing any number of tasks. These algorithms could help explain the missing links.

I'd suggest starting with some of his older work to see they are a credentialed research scientist and not making up 'woo' whole cloth.

bglazer 2 days ago||
I’m curious how you found Michael’s work? Like what led you to familiarize yourself with his research?

He seems to be essentially the only biologist that hackernews knows and he always (always!) comes up in biology discussion. I’m wondering how this particular situation arose.

pixl97 2 days ago||
It's been long enough that I'm not really sure. It's every time I see some of his work the way he looks at problems always intrigued me.

Dogma in science tends to blind people, and when a field starts slowing down and running into things we cannot address then we have to start looking at what we should be questioning.

bglazer 2 days ago||
How do you keep up to date with his work?
pixl97 2 days ago||
Just searching it up in YouTube is a good starting place. He has visited a number of different podcasts. Then from there you can drill down into papers on the interesting things.
bglazer 2 days ago||
Thanks, I was more asking from your perspective.

I’m a working research biologist, and I’m genuinely curious how he became so well known among the hackernews slice of the public. He seems to have hit on some very effective combination of research interests and public communication strategy.

I suspected it was the Lex Fridman podcast appearances, but I wasn’t sure if that was how most people became aware of him.

randomImmigrant 1 day ago||
I think it helps Levin is saying something very different. He’s also much more readable for a computer science crowd since his organizing principles are computation adjacent at least, though he, thankfully, isn’t a computational functionalist in the traditional sense.
rdtsc 2 days ago||
> Demonstrate the emergence of life from chemical precursors in a laboratory setting

Wait, isn’t this an already solved problem? I remember in the 90s school books (material written in the 80s-70s likely) it was presented as “so and so did electric discharges in an atmospheric gas mixtures and they got organic molecules” therefore the origins of life is solved. I remember thinking how cool that was. I guess the “therefore” step was sort of a lie if it’s still an open problem…

Metacelsus 2 days ago||
You're thinking of the Miller-Urey experiment. They got some biomolecules (amino acids, etc) but definitely not life
rdtsc 2 days ago||
Yeah that's the one. Just looked it up it was it the early 1950s. I guess I was either too impressionable or the textbook and/or teacher cut a few corners because I remember it being presented as "we solved the origins of life already" situation.
ricksunny 2 days ago|||
Science was presented in a just-so way in our youth. It still is ( #Scicomms ), but back then, whether reading Junior Scholastic or the margin captions of a high school science textbook handed down ex cathedra, there was no communication pathway to pose vexing questions in a publicly visible way as we do now.

We don't even know if Miller or Urey themselves would have (or did) frame their results that way. The honest framing is 'hey this is one way to form amino acids, and maybe that pathway is reflective of the origin of life, but that supposition remains conjecture on our part.'

NooneAtAll3 2 days ago||
biologists got even further - experiments managed to make RNA longer than a different team's self-replicating RNA

still not all the way to something alive-like

I recently watched this video on the topic https://www.youtube.com/watch?v=OsXGgrXwllc

unknown-unknown 2 days ago|
https://en.wikipedia.org/wiki/Lists_of_problems

https://en.wikipedia.org/wiki/List_of_unsolved_problems_in_b...

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